GCLC Antibody

Product Specifications

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

NCBI Gene ID

2729

Swiss Prot

P48506

Accession Number

P48506

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This GCLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human GCLC.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Obesity, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

73 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

GCLC

NCBI Official Name

Glutamate--cysteine ligase catalytic subunit

NCBI Organism

Homo sapiens

Background Reference 01

Man, B.L., et al.Clin Neurosci 17 (10) :1244-1247 (2010)

Background Reference 02

Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :

Background Reference 03

Le, T.M., et al. Mol. Genet. Metab. 101 (1) :55-61 (2010)

Background Reference 04

Fullerton, J.M., et al. Bipolar Disord 12 (5) :550-556 (2010)

Other Product Names

Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GCLC, GLCL, GLCLC

Tested Applications

Protein ID

1346190

Physical Properties

Liquid

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

EKE62675 96 Tests

Bovine TG (Thyroglobulin) ELISA Kit

Ask

View Details

MBS651054-01 0.005 mg

TACI, Recombinant, Human (Transmembrane activator and CAML interactor)

Ask

View Details

MBS651054-02 0.02 mg

TACI, Recombinant, Human (Transmembrane activator and CAML interactor)

Ask

View Details

MBS651054-03 5x 0.02 mg

TACI, Recombinant, Human (Transmembrane activator and CAML interactor)

Ask

View Details