EpCAM Antibody

Product Specifications

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

NCBI Gene ID

4072

Swiss Prot

P16422

Accession Number

P16422

Host

Rabbit

Reactivity

Human

Immunogen

This EpCAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-229 amino acids from the C-terminal region of human EpCAM.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

35 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

EPCAM

NCBI Official Name

Epithelial cell adhesion molecule

NCBI Organism

Homo sapiens

Background Reference 01

Kimura, O., et al. Cancer Sci. 101 (10) :2145-2155 (2010)

Background Reference 02

Jiang, L., et al. Breast Cancer Res. Treat. (2010) In press :

Background Reference 03

Lugli, A., et al. Br. J. Cancer 103 (3) :382-390 (2010)

Background Reference 04

Johnatty, S.E., et al. PLoS Genet. (7), E1001016 (2010) :

Other Product Names

Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1