NTAN1 Antibody

Product Specifications

Background

Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH) . Conversion of the resulting N-terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position (By similarity) .

NCBI Gene ID

123803

Swiss Prot

Q96AB6

Accession Number

Q96AB6

Host

Rabbit

Reactivity

Human

Immunogen

This NTAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-230 amino acids from the C-terminal region of human NTAN1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

35 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

NTAN1

NCBI Official Name

Protein N-terminal asparagine amidohydrolase

NCBI Organism

Homo sapiens

Background Reference 01

Okada, Y., et al. Hum. Mol. Genet. 19 (11) :2303-2312 (2010)

Background Reference 02

Kamdem, L.K., et al. Pharmacogenet. Genomics 18 (6) :507-514 (2008)

Background Reference 03

Lamesch, P., et al. Genomics 89 (3) :307-315 (2007)

Background Reference 04

Grigoryev, S., et al. J. Biol. Chem. 271 (45) :28521-28532 (1996)

Other Product Names

Protein N-terminal asparagine amidohydrolase, 351-, Protein NH2-terminal asparagine amidohydrolase, PNAA, Protein NH2-terminal asparagine deamidase, PNAD, Protein N-terminal Asn amidase, Protein N-terminal asparagine amidase, Protein NTN-amidase, NTAN1