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MRPL54 Antibody

Product Specifications

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq].

NCBI Gene ID

116541

Swiss Prot

Q6P161

Accession Number

Q6P161

Host

Rabbit

Reactivity

Human

Immunogen

This MRPL54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the Central region of human MRPL54.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

16 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

MRPL54

NCBI Official Name

39S ribosomal protein L54, mitochondrial

NCBI Organism

Homo sapiens

Background Reference 01

Lamesch, P., et al. Genomics 89 (3) :307-315 (2007)

Background Reference 02

Zhang, Z., et al. Genomics 81 (5) :468-480 (2003)

Background Reference 03

Koc, E.C., et al. J. Biol. Chem. 276 (47) :43958-43969 (2001)

Background Reference 04

Adams, M.D., et al. Nature 377 (6547 SUPPL), 3-174 (1995) :

Other Product Names

39S ribosomal protein L54, mitochondrial, L54mt, MRP-L54, MRPL54

Tested Applications

WB

Protein ID

74737109

Physical Properties

Liquid

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