GTF2A1 Antibody

Product Specifications

Background

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]) . One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.

NCBI Gene ID

2957

Swiss Prot

P52655

Accession Number

P52655

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This GTF2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the C-terminal region of human GTF2A1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

42 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

GTF2A1

NCBI Official Name

Transcription initiation factor IIA subunit 1

NCBI Organism

Homo sapiens

Background Reference 01

Huang, Y.W., et al. Oncol. Rep. 22 (4) :853-861 (2009)

Background Reference 02

Vega, A., et al. Gynecol. Oncol. 112 (1) :210-214 (2009)

Background Reference 03

Hieb, A.R., et al. J. Mol. Biol. 372 (3) :619-632 (2007)

Background Reference 04

Lee, K.A., et al. Cytokine 38 (2) :96-100 (2007)

Other Product Names

Transcription initiation factor IIA subunit 1, General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42, Transcription initiation factor IIA alpha chain, TFIIA p35 subunit, Transcription initiation factor IIA beta chain, TFIIA p19 subunit, GTF2A1, TF2A1