PDZD3 Antibody

Product Specifications

Background

Guanylyl cyclase C (GCC, or GUCY2C; MIM 601330) produces cGMP following the binding of either endogenous ligands or heat-stable enterotoxins secreted by E. coli and other enteric bacteria. Activation of GCC initiates a signaling cascade that leads to phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR; MIM 602421), followed by a net efflux of ions and water into the intestinal lumen. IKEPP is a regulatory protein that associates with GCC and regulates the amount of cGMP produced following receptor stimulation (Scott et al., 2002 [PubMed 11950846]) .

NCBI Gene ID

79849

Swiss Prot

Q86UT5

Accession Number

Q86UT5

Host

Rabbit

Reactivity

Human

Immunogen

This PDZD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-285 amino acids from the Central region of human PDZD3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

61 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PDZD3

NCBI Official Name

Na (+) /H (+) exchange regulatory cofactor NHE-RF4

NCBI Organism

Homo sapiens

Background Reference 01

Zachos, N.C., et al. Cell. Physiol. Biochem. 22 (5-6), 693-704 (2008) :

Background Reference 02

Kato, Y., et al. Mol. Pharmacol. 67 (3) :734-743 (2005)

Background Reference 03

Hegedus, T., et al. Biochem. Biophys. Res. Commun. 302 (3) :454-461 (2003)

Background Reference 04

Scott, R.O., et al. J. Biol. Chem. 277 (25) :22934-22941 (2002)

Other Product Names

Na (+) /H (+) exchange regulatory cofactor NHE-RF4, NHERF-4, Intestinal and kidney-enriched PDZ protein, Natrium-phosphate cotransporter IIa C-terminal-associated protein 2, Na/Pi cotransporter C-terminal-associated protein 2, NaPi-Cap2, PDZ domain-containing protein 2, PDZ domain-containing protein 3, Sodium-hydrogen exchanger regulatory factor 4, PDZD3, IKEPP, NHERF4, PDZK2