SPO11 Antibody
Product Specifications
Background
Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described.
NCBI Gene ID
23626
Swiss Prot
Q9Y5K1
Accession Number
Q9Y5K1
Host
Rabbit
Reactivity
Human
Immunogen
This SPO11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-326 amino acids from the C-terminal region of human SPO11.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
45 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
SPO11
NCBI Official Name
Meiotic recombination protein SPO11
NCBI Organism
Homo sapiens
Background Reference 01
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 02
Aston, K.I., et al. Hum. Reprod. 25 (6) :1383-1397 (2010)
Background Reference 03
Mandon-Pepin, B., et al. Eur. J. Endocrinol. 158 (1) :107-115 (2008)
Background Reference 04
Christensen, G.L., et al. Fertil. Steril. 84 (3) :758-760 (2005)
Other Product Names
Meiotic recombination protein SPO11, Cancer/testis antigen 35, CT35, SPO11
Tested Applications
WB
Protein ID
7674367
Physical Properties
Liquid
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