MARCH10 Antibody

Product Specifications

Background

MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19) . MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]) .

NCBI Gene ID

162333

Swiss Prot

Q8NA82

Accession Number

Q8NA82

Host

Rabbit

Reactivity

Human

Immunogen

This MARCH10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-274 amino acids from the Central region of human MARCH10.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

91 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

MARCH10

NCBI Official Name

Probable E3 ubiquitin-protein ligase MARCH10

NCBI Organism

Homo sapiens

Background Reference 01

Yoshida, T., et al. Int. J. Mol. Med. 25 (4) :649-656 (2010)

Background Reference 02

Oguri, M., et al. Am. J. Hypertens. 23 (1) :70-77 (2010)

Background Reference 03

Yoshida, T., et al. Int. J. Mol. Med. 24 (4) :539-547 (2009)

Background Reference 04

Morokuma, Y., et al. J. Biol. Chem. 282 (34) :24806-24815 (2007)

Other Product Names

Probable E3 ubiquitin-protein ligase MARCH10, 632-, Membrane-associated RING finger protein 10, Membrane-associated RING-CH protein X, MARCH-X, RING finger protein 190, MARCH10, RNF190