POLR2D Antibody

Product Specifications

Background

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.

NCBI Gene ID

5433

Swiss Prot

O15514

Accession Number

O15514

Host

Rabbit

Reactivity

Human

Immunogen

This POLR2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the Central region of human POLR2D.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

16 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

POLR2D

NCBI Official Name

DNA-directed RNA polymerase II subunit RPB4

NCBI Organism

Homo sapiens

Background Reference 01

Michiels, S., et al. Carcinogenesis 30 (5) :763-768 (2009)

Background Reference 02

Meka, H., et al. Nucleic Acids Res. 33 (19) :6435-6444 (2005)

Background Reference 03

Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100 (22) :12666-12671 (2003)

Background Reference 04

Kaehlcke, K., et al. Mol. Cell 12 (1) :167-176 (2003)

Other Product Names

DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, RPB16, POLR2D