POLR1E Antibody

Product Specifications

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity) .

NCBI Gene ID

64425

Swiss Prot

Q9GZS1

Accession Number

Q9GZS1

Host

Rabbit

Reactivity

Human

Immunogen

This POLR1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-198 amino acids from the Central region of human POLR1E.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

54 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

POLR1E

NCBI Official Name

DNA-directed RNA polymerase I subunit RPA49

NCBI Organism

Homo sapiens

Background Reference 01

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Background Reference 02

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

Background Reference 03

Percipalle, P., et al. EMBO Rep. 7 (5) :525-530 (2006)

Background Reference 04

Yamamoto, K., et al. Mol. Cell. Biol. 24 (14) :6338-6349 (2004)

Other Product Names

DNA-directed RNA polymerase I subunit RPA49, RNA polymerase I subunit A49, DNA-directed RNA polymerase I subunit E, RNA polymerase I-associated factor 1, RNA polymerase I-associated factor 53, POLR1E, PAF53, PRAF1