ARF5 Antibody

Product Specifications

Background

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6) . The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq].

NCBI Gene ID

381

Swiss Prot

P84085

Accession Number

P84085

Host

Rabbit

Reactivity

Human

Immunogen

This ARF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-108 amino acids from the Central region of human ARF5.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

21 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

ARF5

NCBI Official Name

ADP-ribosylation factor 5

NCBI Organism

Homo sapiens

Background Reference 01

Shiba, T., et al. Proc. Natl. Acad. Sci. U.S.A. 103 (42) :15416-15421 (2006)

Background Reference 02

Austin, C., et al. Biochemistry 41 (14) :4669-4677 (2002)

Background Reference 03

Shin, O.H., et al. Biochemistry 40 (36) :10846-10852 (2001)

Background Reference 04

Nevrivy, D.J., et al. J. Biol. Chem. 275 (22) :16827-16836 (2000)

Other Product Names

ADP-ribosylation factor 5, ARF5

Tested Applications

Protein ID

51316990

Physical Properties

Liquid

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