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P4HA1 Antibody

Product Specifications

Background

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

NCBI Gene ID

5033

Swiss Prot

P13674

Accession Number

P13674

Host

Rabbit

Reactivity

Human

Immunogen

This P4HA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-442 amino acids from the C-terminal region of human P4HA1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=58-934

Calculated Molecular Weight

61 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

P4HA1

NCBI Official Name

Prolyl 4-hydroxylase subunit alpha-1

NCBI Organism

Homo sapiens

Background Reference 01

Rose, J. Phd, et al. Mol. Med. (2010) In press :

Background Reference 02

Gorres, K.L., et al. PLoS ONE (11), E7635 (2009) :

Background Reference 03

Koivunen, P., et al. J. Biol. Chem. 281 (39) :28712-28720 (2006)

Background Reference 04

Fahling, M., et al. J. Biol. Chem. 281 (36) :26089-26101 (2006)

Other Product Names

Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA1, P4HA

Tested Applications

WB

Protein ID

2507090

Physical Properties

Liquid

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