RAPGEF5 Antibody

Product Specifications

Background

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]) .

NCBI Gene ID

9771

Swiss Prot

Q92565

Accession Number

Q92565

Host

Rabbit

Reactivity

Human

Immunogen

This RAPGEF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 529-555 amino acids from the C-terminal region of human RAPGEF5.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

68 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

RAPGEF5

NCBI Official Name

Rap guanine nucleotide exchange factor 5

NCBI Organism

Homo sapiens

Background Reference 01

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Background Reference 02

Okada, Y., et al. Hum. Mol. Genet. 19 (11) :2303-2312 (2010)

Background Reference 03

Hwang, S.J., et al. BMC Med. Genet.SUPPL 1, S10 (2007) :

Background Reference 04

Rebhun, J.F., et al. J. Biol. Chem. 275 (45) :34901-34908 (2000)

Other Product Names

Rap guanine nucleotide exchange factor 5, Guanine nucleotide exchange factor for Rap1, M-Ras-regulated Rap GEF, MR-GEF, Related to Epac, Repac, RAPGEF5, GFR, KIAA0277, MRGEF