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RPN1 Antibody

Product Specifications

Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.

NCBI Gene ID

6184

Swiss Prot

P04843

Accession Number

P04843

Host

Rabbit

Reactivity

Human

Immunogen

This RPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 559-587 amino acids from the C-terminal region of human RPN1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Monkey, Pig, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

69 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

RPN1

NCBI Official Name

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

NCBI Organism

Homo sapiens

Background Reference 01

Ruiz-Canada, C., et al. Cell 136 (2) :272-283 (2009)

Background Reference 02

Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17 (12) :3558-3566 (2008)

Background Reference 03

Wilson, C.M., et al. Proc. Natl. Acad. Sci. U.S.A. 105 (28) :9534-9539 (2008)

Background Reference 04

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

Other Product Names

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1, RPN1

Tested Applications

WB

Protein ID

132559

Physical Properties

Liquid

Frequently Asked Questions

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