POLR3G Antibody
Product Specifications
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.
NCBI Gene ID
10622
Swiss Prot
O15318
Accession Number
O15318
Host
Rabbit
Reactivity
Human
Immunogen
This POLR3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the C-terminal region of human POLR3G.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=58-852
Calculated Molecular Weight
26 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
POLR3G
NCBI Official Name
DNA-directed RNA polymerase III subunit RPC7
NCBI Organism
Homo sapiens
Background Reference 01
Ablasser, A., et al. Nat. Immunol. 10 (10) :1065-1072 (2009)
Background Reference 02
Chiu, Y.H., et al. Cell 138 (3) :576-591 (2009)
Background Reference 03
Oh, J.H., et al. Mamm. Genome 16 (12) :942-954 (2005)
Background Reference 04
Hu, P., et al. Mol. Cell. Biol. 22 (22) :8044-8055 (2002)
Other Product Names
DNA-directed RNA polymerase III subunit RPC7, RNA polymerase III subunit C7, DNA-directed RNA polymerase III subunit G, RNA polymerase III 32 kDa subunit, RPC32, POLR3G
Tested Applications
WB
Physical Properties
Liquid
Curated Selection
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