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UBE2H Antibody

Product Specifications

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

NCBI Gene ID

7328

Swiss Prot

P62256

Accession Number

P62256

Host

Rabbit

Reactivity

Human

Immunogen

This UBE2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-70 amino acids from the Central region of human UBE2H.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

21 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

UBE2H

NCBI Official Name

Ubiquitin-conjugating enzyme E2 H

NCBI Organism

Homo sapiens

Background Reference 01

Lausen, J., et al. J. Biol. Chem. 285 (8) :5338-5346 (2010)

Background Reference 02

Martin, I., et al. Amyotroph Lateral Scler 10 (5-6), 432-435 (2009) :

Background Reference 03

De Krom, M., et al. Biol. Psychiatry 65 (7) :625-630 (2009)

Background Reference 04

Martin, I., et al. Amyotroph Lateral Scler, 1-4 (2008) In press :

Other Product Names

Ubiquitin-conjugating enzyme E2 H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, UBE2H

Tested Applications

WB

Protein ID

51338683

Physical Properties

Liquid

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