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ATP5D Antibody

Product Specifications

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) . This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified.

NCBI Gene ID

513

Swiss Prot

P30049

Accession Number

P30049

Host

Rabbit

Reactivity

Human

Immunogen

This ATP5D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-157 amino acids from the C-terminal region of human ATP5D.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

17 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

ATP5D

NCBI Official Name

ATP synthase subunit delta, mitochondrial

NCBI Organism

Homo sapiens

Background Reference 01

Grimwood, J., et al. Nature 428 (6982) :529-535 (2004)

Background Reference 02

Itoh, H., et al. Nature 427 (6973) :465-468 (2004)

Background Reference 03

Cross, R.L. Nature 427 (6973) :407-408 (2004)

Background Reference 04

Hong, S., et al. J. Bioenerg. Biomembr. 35 (2) :95-120 (2003)

Other Product Names

ATP synthase subunit delta, mitochondrial, F-ATPase delta subunit, ATP5D

Tested Applications

WB

Protein ID

584812

Physical Properties

Liquid

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