EIF3K Antibody

Product Specifications

Background

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]) .

NCBI Gene ID

27335

Swiss Prot

Q9UBQ5

Accession Number

Q9UBQ5

Host

Rabbit

Reactivity

Human

Immunogen

This EIF3K antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-204 amino acids from the C-terminal region of human EIF3K.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

25 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

EIF3K

NCBI Official Name

Eukaryotic translation initiation factor 3 subunit K {ECO:0000255|HAMAP-Rule:MF_03010}

NCBI Organism

Homo sapiens

Background Reference 01

Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105 (47) :18139-18144 (2008)

Background Reference 02

Lin, Y.M., et al. J. Cell. Sci. 121 (PT 14), 2382-2393 (2008) :

Background Reference 03

Masutani, M., et al. EMBO J. 26 (14) :3373-3383 (2007)

Background Reference 04

Damoc, E., et al. Mol. Cell Proteomics 6 (7) :1135-1146 (2007)

Other Product Names

Eukaryotic translation initiation factor 3 subunit K {ECO:0000255|HAMAP-Rule:MF_03010}, eIF3k {ECO:0000255|HAMAP-Rule:MF_03010}, Eukaryotic translation initiation factor 3 subunit 12 {ECO:0000255|HAMAP-Rule:MF_03010}, Muscle-specific gene M9 protein, PLAC-24, eIF-3 p25 {ECO:0000255|HAMAP-Rule:MF_03010}, eIF-3 p28, EIF3K {ECO:0000255|HAMAP-Rule:MF_03010}