USP16 Antibody

Product Specifications

Background

This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

NCBI Gene ID

10600

Swiss Prot

Q9Y5T5

Accession Number

Q9Y5T5

Host

Rabbit

Reactivity

Human

Immunogen

This USP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-160 amino acids from the N-terminal region of human USP16.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Monkey

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

94 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

USP16

NCBI Official Name

Ubiquitin carboxyl-terminal hydrolase 16 {ECO:0000255|HAMAP-Rule:MF_03062}

NCBI Organism

Homo sapiens

Background Reference 01

Gelsi-Boyer, V., et al. BMC Cancer 8, 299 (2008) :

Background Reference 02

Joo, H.Y., et al. Nature 449 (7165) :1068-1072 (2007)

Background Reference 03

Pai, M.T., et al. J. Mol. Biol. 370 (2) :290-302 (2007)

Background Reference 04

Sugiyama, N., et al. Mol. Cell Proteomics 6 (6) :1103-1109 (2007)

Other Product Names

Ubiquitin carboxyl-terminal hydrolase 16 {ECO:0000255|HAMAP-Rule:MF_03062}, 341912 {ECO:0000255|HAMAP-Rule:MF_03062}, Deubiquitinating enzyme 16 {ECO:0000255|HAMAP-Rule:MF_03062}, Ubiquitin thioesterase 16 {ECO:0000255|HAMAP-Rule:MF_03062}, Ubiquitin-processing protease UBP-M, Ubiquitin-specific-processing protease 16 {ECO:0000255|HAMAP-Rule:MF_03062}, USP16 {ECO:0000255|HAMAP-Rule:MF_03062}