MED16 Antibody

Product Specifications

Background

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

NCBI Gene ID

10025

Swiss Prot

Q9Y2X0

Accession Number

Q9Y2X0

Host

Rabbit

Reactivity

Human

Immunogen

This MED16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human MED16.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

97 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

MED16

NCBI Official Name

Mediator of RNA polymerase II transcription subunit 16

NCBI Organism

Homo sapiens

Background Reference 01

Sato, S., et al. Mol. Cell 14 (5) :685-691 (2004)

Background Reference 02

Tomomori-Sato, C., et al. J. Biol. Chem. 279 (7) :5846-5851 (2004)

Background Reference 03

Wang, Q., et al. J. Biol. Chem. 277 (45) :42852-42858 (2002)

Background Reference 04

Kang, Y.K., et al. Proc. Natl. Acad. Sci. U.S.A. 99 (5) :2642-2647 (2002)

Other Product Names

Mediator of RNA polymerase II transcription subunit 16, Mediator complex subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, Trap95, Vitamin D3 receptor-interacting protein complex 92 kDa component, DRIP92, MED16, DRIP92 {ECO:0000312|EMBL:AAD310871}, THRAP5