SIGLEC5 Antibody

Product Specifications

Background

The sialic acid-binding immunoglobulin-like lectins (SIGLECs), such as SIGLEC5, are a subgroup of the immunoglobulin (Ig) superfamily that mediate protein-carbohydrate interactions. They specifically interact with sialic acids in glycoproteins and glycolipids, with each SIGLEC having a particular preference for both the nature of the sialic acid and its glycosidic linkage to adjacent sugars. SIGLECs have similar structures, including extracellular Ig-like domains composed of an N-terminal V-set domain followed by varying numbers of C2-set domains. It appears that all SIGLECs have an unusual arrangement of conserved cysteine residues in the V-set and adjacent C2-set domains. Most SIGLECs are expressed uniquely within the hematopoietic system (Cornish et al., 1998 [PubMed 9731071]) .

NCBI Gene ID

8778

Swiss Prot

O15389

Accession Number

O15389

Host

Rabbit

Reactivity

Human

Immunogen

This SIGLEC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-132 amino acids from the N-terminal region of human SIGLEC5.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

61 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

SIGLEC5

NCBI Official Name

Sialic acid-binding Ig-like lectin 5

NCBI Organism

Homo sapiens

Background Reference 01

Soto, P.C., et al. J. Immunol. 184 (8) :4185-4195 (2010)

Background Reference 02

Davila, S., et al. Genes Immun. 11 (3) :232-238 (2010)

Background Reference 03

Carlin, A.F., et al. J. Exp. Med. 206 (8) :1691-1699 (2009)

Background Reference 04

Zhuravleva, M.A., et al. J. Mol. Biol. 375 (2) :437-447 (2008)

Other Product Names

Sialic acid-binding Ig-like lectin 5, Siglec-5, CD33 antigen-like 2, Obesity-binding protein 2, OB-BP2, OB-binding protein 2, CD170, SIGLEC5, CD33L2, OBBP2