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DDX28 Antibody

Product Specifications

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq].

NCBI Gene ID

55794

Swiss Prot

Q9NUL7

Accession Number

Q9NUL7

Host

Rabbit

Reactivity

Human

Immunogen

This DDX28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 370-398 amino acids from the C-terminal region of human DDX28.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=58-493

Calculated Molecular Weight

60 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

DDX28

NCBI Official Name

Probable ATP-dependent RNA helicase DDX28

NCBI Organism

Homo sapiens

Background Reference 01

Bogenhagen, D.F., et al. J. Biol. Chem. 283 (6) :3665-3675 (2008)

Background Reference 02

Valgardsdottir, R., et al. J. Biol. Chem. 278 (23) :21146-21154 (2003)

Background Reference 03

Valgardsdottir, R., et al. J. Biol. Chem. 276 (34) :32056-32063 (2001)

Background Reference 04

Simpson, J.C., et al. EMBO Rep. 1 (3) :287-292 (2000)

Other Product Names

Probable ATP-dependent RNA helicase DDX28, Mitochondrial DEAD box protein 28, DDX28, MDDX28

Tested Applications

WB

Protein ID

296434476

Physical Properties

Liquid

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