EIF3C Antibody

Product Specifications

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

NCBI Gene ID

8663

Swiss Prot

Q99613

Accession Number

Q99613

Host

Rabbit

Reactivity

Mouse

Immunogen

This EIF3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 538-566 amino acids from the Central region of human EIF3C.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Rat, Xenopus, Human

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

105 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

EIF3C

NCBI Official Name

Eukaryotic translation initiation factor 3 subunit C {ECO:0000255|HAMAP-Rule:MF_03002}

NCBI Organism

Homo sapiens

Background Reference 01

Imielinski, M., et al. Nat. Genet. 41 (12) :1335-1340 (2009)

Background Reference 02

Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105 (47) :18139-18144 (2008)

Background Reference 03

Masutani, M., et al. EMBO J. 26 (14) :3373-3383 (2007)

Background Reference 04

Damoc, E., et al. Mol. Cell Proteomics 6 (7) :1135-1146 (2007)

Other Product Names

Eukaryotic translation initiation factor 3 subunit C {ECO:0000255|HAMAP-Rule:MF_03002}, eIF3c {ECO:0000255|HAMAP-Rule:MF_03002}, Eukaryotic translation initiation factor 3 subunit 8 {ECO:0000255|HAMAP-Rule:MF_03002}, eIF3 p110 {ECO:0000255|HAMAP-Rule:MF_03002}, EIF3C {ECO:0000255|HAMAP-Rule:MF_03002}