MED17 Antibody

Product Specifications

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

NCBI Gene ID

9440

Swiss Prot

Q9NVC6

Accession Number

Q9NVC6

Host

Rabbit

Reactivity

Human

Immunogen

This MED17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 332-361 amino acids from the Central region of human MED17.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

73 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

MED17

NCBI Official Name

Mediator of RNA polymerase II transcription subunit 17

NCBI Organism

Homo sapiens

Background Reference 01

Matsuoka, S., et al. Science 316 (5828) :1160-1166 (2007)

Background Reference 02

Jang, M.K., et al. Mol. Cell 19 (4) :523-534 (2005)

Background Reference 03

Tomomori-Sato, C., et al. J. Biol. Chem. 279 (7) :5846-5851 (2004)

Background Reference 04

Sato, S., et al. J. Biol. Chem. 278 (17) :15123-15127 (2003)

Other Product Names

Mediator of RNA polymerase II transcription subunit 17, Activator-recruited cofactor 77 kDa component, ARC77, Cofactor required for Sp1 transcriptional activation subunit 6, CRSP complex subunit 6, Mediator complex subunit 17, Thyroid hormone receptor-associated protein complex 80 kDa component, Trap80, Transcriptional coactivator CRSP77, Vitamin D3 receptor-interacting protein complex 80 kDa component, DRIP80, MED17, ARC77, CRSP6, DRIP77, DRIP80, TRAP80