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HMGCL Antibody

Product Specifications

Background

HMGCL belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

NCBI Gene ID

3155

Swiss Prot

P35914

Accession Number

P35914

Host

Rabbit

Reactivity

Human

Immunogen

This HMGCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from the Central region of human HMGCL.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Obesity, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Monkey

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

34 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

HMGCL

NCBI Official Name

Hydroxymethylglutaryl-CoA lyase, mitochondrial

NCBI Organism

Homo sapiens

Background Reference 01

Fu, Z., et al. J. Biol. Chem. 285 (34) :26341-26349 (2010)

Background Reference 02

Pierron, S., et al. Arch Pediatr 17 (1) :10-13 (2010)

Background Reference 03

Menao, S., et al. Hum. Mutat. 30 (3), E520-E529 (2009) :

Background Reference 04

Lin, W.D., et al. Clin. Chim. Acta 401 (1-2), 33-36 (2009) :

Other Product Names

Hydroxymethylglutaryl-CoA lyase, mitochondrial, HL, HMG-CoA lyase, 3-hydroxy-3-methylglutarate-CoA lyase, HMGCL

Tested Applications

WB

Protein ID

24418852

Physical Properties

Liquid

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