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PIGT Antibody

Product Specifications

Background

This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI) -anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Several transcript variants encoding different isoforms have been found for this gene.

NCBI Gene ID

51604

Swiss Prot

Q969N2

Accession Number

Q969N2

Host

Rabbit

Reactivity

Human

Immunogen

This PIGT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 392-421 amino acids from the C-terminal region of human PIGT.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

66 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PIGT

NCBI Official Name

GPI transamidase component PIG-T

NCBI Organism

Homo sapiens

Background Reference 01

Li, H.L., et al. FEBS Lett. 580 (7) :1723-1728 (2006)

Background Reference 02

Vainauskas, S., et al. J. Biol. Chem. 280 (16) :16402-16409 (2005)

Background Reference 03

Ohishi, K., et al. J. Biol. Chem. 278 (16) :13959-13967 (2003)

Background Reference 04

Eisenhaber, B., et al. Bioessays 25 (4) :367-385 (2003)

Other Product Names

GPI transamidase component PIG-T, Phosphatidylinositol-glycan biosynthesis class T protein, PIGT

Tested Applications

WB

Protein ID

44888284

Physical Properties

Liquid

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