TAF1A Antibody

Product Specifications

Background

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.

NCBI Gene ID

9015

Swiss Prot

Q15573

Accession Number

Q15573

Host

Rabbit

Reactivity

Human

Immunogen

This TAF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the N-terminal region of human TAF1A.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

53 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

TAF1A

NCBI Official Name

TATA box-binding protein-associated factor RNA polymerase I subunit A

NCBI Organism

Homo sapiens

Background Reference 01

Greco-Stewart, V.S., et al. Virology 386 (1) :12-15 (2009)

Background Reference 02

Yamamoto, K., et al. Mol. Cell. Biol. 24 (14) :6338-6349 (2004)

Background Reference 03

Dynes, J.L., et al. J. Biochem. 135 (3) :429-438 (2004)

Background Reference 04

Hirschler-Laszkiewicz, I., et al. J. Biol. Chem. 278 (21) :18953-18959 (2003)

Other Product Names

TATA box-binding protein-associated factor RNA polymerase I subunit A, RNA polymerase I-specific TBP-associated factor 48 kDa, TAFI48, TATA box-binding protein-associated factor 1A, TBP-associated factor 1A, Transcription factor SL1, Transcription initiation factor SL1/TIF-IB subunit A, TAF1A