NME1 Antibody

Product Specifications

Background

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

NCBI Gene ID

4830

Swiss Prot

P15531

Accession Number

P15531

Host

Rabbit

Reactivity

Human

Immunogen

This NME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the Central region of human NME1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

17 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

NME1

NCBI Official Name

Nucleoside diphosphate kinase A

NCBI Organism

Homo sapiens

Background Reference 01

Boissan, M., et al. Cancer Res. 70 (19) :7710-7722 (2010)

Background Reference 02

Wang, P.H., et al. Gynecol. Oncol. 119 (1) :70-75 (2010)

Background Reference 03

Conery, A.R., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (35) :15461-15466 (2010)

Background Reference 04

Wang, Z., et al. Med. Sci. Monit. 16 (8), CR357-CR364 (2010) :

Other Product Names

Nucleoside diphosphate kinase A, NDK A, NDP kinase A, Granzyme A-activated DNase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NME1, NDPKA, NM23