CDC2 Antibody

Product Specifications

Background

CDC2 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of CDC2 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.

NCBI Gene ID

983

Swiss Prot

P06493

Accession Number

P06493

Host

Rabbit

Reactivity

Human

Immunogen

This CDC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-241 amino acids from the C-terminal region of human CDC2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle, Neuroscience

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Xenopus, Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

34 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

CDK1

NCBI Official Name

Cyclin-dependent kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Forester, C.M., Proc. Natl. Acad. Sci. U.S.A. 104 (50), 19867-19872 (2007)

Background Reference 02

Skoufias, D.A., J. Cell Biol. 179 (4), 671-685 (2007)

Background Reference 03

Hester, K.D., Cell Cycle (13), 1594-1604 (2007)

Other Product Names

Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, CDK1, CDC2, CDC28A, CDKN1, P34CDC2