NEDD4L Antibody

Product Specifications

Background

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK.

NCBI Gene ID

23327

Swiss Prot

Q96PU5

Accession Number

Q96PU5

Host

Rabbit

Reactivity

Mouse

Immunogen

This NEDD4L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-604 amino acids from the Central region of human NEDD4L.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

112 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

NEDD4L

NCBI Official Name

E3 ubiquitin-protein ligase NEDD4-like

NCBI Organism

Homo sapiens

Background Reference 01

Zhou, R., et al. J. Biol. Chem. 285 (40) :30523-30530 (2010)

Background Reference 02

Raikwar, N.S., et al. Am. J. Physiol. Renal Physiol. 299 (2), F436-F444 (2010) :

Background Reference 03

Hallows, K.R., et al. J. Biol. Chem. 285 (28) :21671-21678 (2010)

Background Reference 04

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Other Product Names

E3 ubiquitin-protein ligase NEDD4-like, 632-, NEDD42, Nedd4-2, NEDD4L, KIAA0439, NEDL3

Tested Applications

Protein ID

73921204

Physical Properties

Liquid

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