BRF1 Antibody

Product Specifications

Background

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Two alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. Other transcript variants are possible, but their full-length natures have not been completely characterized. [provided by RefSeq].

NCBI Gene ID

2972

Swiss Prot

Q92994

Accession Number

Q92994

Host

Rabbit

Reactivity

Human

Immunogen

This BRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-342 amino acids from the Central region of human BRF1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

74 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

BRF1

NCBI Official Name

Transcription factor IIIB 90 kDa subunit

NCBI Organism

Homo sapiens

Background Reference 01

Haurie, V., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (9) :4176-4181 (2010)

Background Reference 02

Maitra, S., et al. RNA 14 (5) :950-959 (2008)

Background Reference 03

Leal, J.F., et al. Oncogene 27 (14) :1961-1970 (2008)

Background Reference 04

Yoo, N.K., et al. Cell. Microbiol. 10 (3) :583-592 (2008)

Other Product Names

Transcription factor IIIB 90 kDa subunit, TFIIIB90, hTFIIIB90, B-related factor 1, BRF-1, hBRF, TAF3B2, TATA box-binding protein-associated factor, RNA polymerase III, subunit 2, BRF1, BRF, GTF3B, TAF3B2, TAF3C