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COBRA1 Antibody

Product Specifications

Background

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040) . NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]) .

NCBI Gene ID

25920

Swiss Prot

Q8WX92

Accession Number

Q8WX92

Host

Rabbit

Reactivity

Human

Immunogen

This COBRA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the Central region of human COBRA1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=57-973

Calculated Molecular Weight

66 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

NELFB

NCBI Official Name

Negative elongation factor B

NCBI Organism

Homo sapiens

Background Reference 01

Sun, J., et al. J. Cell. Biochem. 103 (6) :1798-1807 (2008)

Background Reference 02

Sun, J., et al. J. Steroid Biochem. Mol. Biol. 107 (3-5), 131-139 (2007) :

Background Reference 03

Wu, C., et al. Proteomics 7 (11) :1775-1785 (2007)

Background Reference 04

Narita, T., et al. Mol. Cell 26 (3) :349-365 (2007)

Other Product Names

Negative elongation factor B, NELF-B, Cofactor of BRCA1, NELFB, COBRA1, KIAA1182

Tested Applications

WB

Protein ID

38372378

Physical Properties

Liquid

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