POLR2B Antibody

Product Specifications

Background

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq].

NCBI Gene ID

5431

Swiss Prot

P30876

Accession Number

P30876

Host

Rabbit

Reactivity

Human

Immunogen

This POLR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 797-826 amino acids from the Central region of human POLR2B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

134 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

POLR2B

NCBI Official Name

DNA-directed RNA polymerase II subunit RPB2

NCBI Organism

Homo sapiens

Background Reference 01

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Background Reference 02

Michiels, S., et al. Carcinogenesis 30 (5) :763-768 (2009)

Background Reference 03

Tu, S., et al. Nat. Struct. Mol. Biol. 15 (4) :419-421 (2008)

Background Reference 04

Lee, J.H., et al. Mol. Cell. Biol. 28 (2) :609-618 (2008)

Other Product Names

DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II 140 kDa polypeptide, DNA-directed RNA polymerase II subunit B, RNA polymerase II subunit 2, RNA polymerase II subunit B2, POLR2B