Mouse Aurka Antibody

Product Specifications

Background

Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity) . Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.

NCBI Gene ID

20878

Swiss Prot

P97477

Accession Number

P97477

Host

Rabbit

Reactivity

Mouse

Immunogen

This Mouse Aurka antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of mouse Aurka.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

45 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

Aurka

NCBI Official Name

Aurora kinase A

NCBI Organism

Mus musculus

Background Reference 01

Kinzel, D., et al. Dev. Cell 19 (1) :66-77 (2010)

Background Reference 02

Van Horn, R.D., et al. J. Biol. Chem. 285 (28) :21849-21857 (2010)

Background Reference 03

Mori, D., et al. Nat. Cell Biol. 11 (9) :1057-1068 (2009)

Background Reference 04

Li, C.C., et al. Mol. Cancer Res. 7 (5) :678-688 (2009)

Other Product Names

Aurora kinase A, Aurora 2, Aurora family kinase 1, Aurora/IPL1-related kinase 1, ARK-1, Aurora-related kinase 1, Ipl1- and aurora-related kinase 1, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase Ayk1, Serine/threonine-protein kinase aurora-A, Aurka