PSMA1 Antibody

Product Specifications

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

NCBI Gene ID

5682

Swiss Prot

P25786

Accession Number

P25786

Host

Rabbit

Reactivity

Human

Immunogen

This PSMA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-262 amino acids from the C-terminal region of human PSMA1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Monkey

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

30 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PSMA1

NCBI Official Name

Proteasome subunit alpha type-1

NCBI Organism

Homo sapiens

Background Reference 01

Zhang, Y., et al. Biochem. Biophys. Res. Commun. 355 (1) :245-251 (2007)

Background Reference 02

Apcher, G.S., et al. FEBS Lett. 569 (1-3), 211-216 (2004) :

Background Reference 03

Jayarapu, K., et al. Biochem. Biophys. Res. Commun. 314 (2) :523-528 (2004)

Background Reference 04

Apcher, G.S., et al. FEBS Lett. 553 (1-2), 200-204 (2003) :

Other Product Names

Proteasome subunit alpha type-1, 30 kDa prosomal protein, PROS-30, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome component C2, Proteasome nu chain, PSMA1, HC2, NU, PROS30, PSC2