HSD3B1 Antibody

Product Specifications

Background

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta (5) -ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.

NCBI Gene ID

3283

Swiss Prot

P14060

Accession Number

P14060

Host

Rabbit

Reactivity

Human

Immunogen

This HSD3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human HSD3B1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle, Obesity, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

42 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

HSD3B1

NCBI Official Name

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

NCBI Organism

Homo sapiens

Background Reference 01

Canzian, F., et al. Hum. Mol. Genet. 19 (19) :3873-3884 (2010)

Background Reference 02

Shimodaira, M., et al. Eur. J. Endocrinol. 163 (4) :671-680 (2010)

Background Reference 03

Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

Background Reference 04

Liu, C.Y., et al. Carcinogenesis 31 (7) :1259-1263 (2010)

Other Product Names

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, Trophoblast antigen FDO161G, 3-beta-hydroxy-Delta (5) -steroid dehydrogenase, 3-beta-hydroxy-5-ene steroid dehydrogenase, Progesterone reductase, Steroid Delta-isomerase, Delta-5-3-ketosteroid isomerase, HSD3B1, 3BH, HSDB3A