POLI Antibody
Product Specifications
Background
Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
NCBI Gene ID
11201
Swiss Prot
Q9UNA4
Accession Number
Q9UNA4
Host
Rabbit
Reactivity
Human
Immunogen
This POLI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 673-701 amino acids from the C-terminal region of human POLI.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
83 kDa
Fragment
Rabbit Ig
Symbol
POLI
NCBI Official Name
DNA polymerase iota
NCBI Organism
Homo sapiens
Background Reference 01
Arora, M., et al. Leukemia 24 (8) :1470-1475 (2010)
Background Reference 02
Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16 (8) :1084-1089 (2010)
Background Reference 03
Kazakov, A.A., et al. Biochemistry Mosc. 75 (7) :905-911 (2010)
Background Reference 04
Liu, C.Y., et al. Carcinogenesis 31 (7) :1259-1263 (2010)
Other Product Names
DNA polymerase iota, Eta2, RAD30 homolog B, POLI, RAD30B
Tested Applications
WB, IHC-P
Protein ID
327478565
Physical Properties
Liquid
Curated Selection
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