USP20 Antibody

Product Specifications

Background

Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2) . Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains.

NCBI Gene ID

10868

Swiss Prot

Q9Y2K6

Accession Number

Q9Y2K6

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This USP20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310-339 amino acids from the Central region of human USP20.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

102 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

USP20

NCBI Official Name

Ubiquitin carboxyl-terminal hydrolase 20

NCBI Organism

Homo sapiens

Background Reference 01

Yoshida, T., et al. Int. J. Mol. Med. 25 (4) :649-656 (2010)

Background Reference 02

Oguri, M., et al. Am. J. Hypertens. 23 (1) :70-77 (2010)

Background Reference 03

Berthouze, M., et al. EMBO J. 28 (12) :1684-1696 (2009)

Background Reference 04

Olsen, J.V., et al. Cell 127 (3) :635-648 (2006)

Other Product Names

Ubiquitin carboxyl-terminal hydrolase 20, Deubiquitinating enzyme 20, Ubiquitin thioesterase 20, Ubiquitin-specific-processing protease 20, VHL-interacting deubiquitinating enzyme 2, hVDU2, USP20, KIAA1003, LSFR3A, VDU2