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B3GALT2 Antibody

Product Specifications

Background

This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine) . The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5) . This gene encodes a protein that functions in N-linked glycoprotein glycosylation and shows strict donor substrate specificity for UDP-galactose.

NCBI Gene ID

8707

Swiss Prot

O43825

Accession Number

O43825

Host

Rabbit

Reactivity

Mouse

Immunogen

This B3GALT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-422 amino acids from the C-terminal region of human B3GALT2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

49 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

B3GALT2

NCBI Official Name

Beta-1,3-galactosyltransferase 2

NCBI Organism

Homo sapiens

Background Reference 01

Lamesch, P., et al. Genomics 89 (3) :307-315 (2007)

Background Reference 02

Sood, R., et al. Genomics 73 (2) :211-222 (2001)

Background Reference 03

Amado, M., et al. Biochim. Biophys. Acta 1473 (1) :35-53 (1999)

Background Reference 04

Amado, M., et al. J. Biol. Chem. 273 (21) :12770-12778 (1998)

Other Product Names

Beta-1,3-galactosyltransferase 2, Beta-1,3-GalTase 2, Beta3Gal-T2, Beta3GalT2, 241-, UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase 2, B3GALT2

Tested Applications

WB

Physical Properties

Liquid

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