POLR2E Antibody

Product Specifications

Background

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

NCBI Gene ID

5434

Swiss Prot

P19388

Accession Number

P19388

Host

Rabbit

Reactivity

Human

Immunogen

This POLR2E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human POLR2E.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

25 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

POLR2E

NCBI Official Name

DNA-directed RNA polymerases I, II, and III subunit RPABC1

NCBI Organism

Homo sapiens

Background Reference 01

Hosgood, H.D. III, et al. Occup Environ Med 66 (12) :848-853 (2009)

Background Reference 02

Cloutier, P., et al. Methods 48 (4) :381-386 (2009)

Background Reference 03

Michiels, S., et al. Carcinogenesis 30 (5) :763-768 (2009)

Background Reference 04

Lamesch, P., et al. Genomics 89 (3) :307-315 (2007)

Other Product Names

DNA-directed RNA polymerases I, II, and III subunit RPABC1, RNA polymerases I, II, and III subunit ABC1, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog, XAP4, POLR2E