POLR2E Antibody
Product Specifications
Background
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.
NCBI Gene ID
5434
Swiss Prot
P19388
Accession Number
P19388
Host
Rabbit
Reactivity
Human
Immunogen
This POLR2E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human POLR2E.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
25 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
POLR2E
NCBI Official Name
DNA-directed RNA polymerases I, II, and III subunit RPABC1
NCBI Organism
Homo sapiens
Background Reference 01
Hosgood, H.D. III, et al. Occup Environ Med 66 (12) :848-853 (2009)
Background Reference 02
Cloutier, P., et al. Methods 48 (4) :381-386 (2009)
Background Reference 03
Michiels, S., et al. Carcinogenesis 30 (5) :763-768 (2009)
Background Reference 04
Lamesch, P., et al. Genomics 89 (3) :307-315 (2007)
Other Product Names
DNA-directed RNA polymerases I, II, and III subunit RPABC1, RNA polymerases I, II, and III subunit ABC1, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog, XAP4, POLR2E
Tested Applications
WB
Protein ID
116242767
Physical Properties
Liquid
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