RPA1 Antibody

Product Specifications

Background

RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA) . aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

NCBI Gene ID

6117

Swiss Prot

P27694

Accession Number

P27694

Host

Rabbit

Reactivity

Human

Immunogen

This RPA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-559 amino acids from the C-terminal region of human RPA1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

68 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

RPA1

NCBI Official Name

Replication protein A 70 kDa DNA-binding subunit

NCBI Organism

Homo sapiens

Background Reference 01

Choi, J.H., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (31) :13660-13665 (2010)

Background Reference 02

Locatelli, G.A., et al. Biochem. J. 429 (3) :573-582 (2010)

Background Reference 03

Briggs, F.B., et al. Am. J. Epidemiol. 172 (2) :217-224 (2010)

Background Reference 04

Guillem, V.M., et al. Am. J. Hematol. 85 (7) :482-486 (2010)

Other Product Names

Replication protein A 70 kDa DNA-binding subunit, RP-A p70, Replication factor A protein 1, RF-A protein 1, Single-stranded DNA-binding protein, Replication protein A 70 kDa DNA-binding subunit, N-terminally processed, RPA1, REPA1, RPA70