MARCH1 Antibody

Product Specifications

Background

MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19) . MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]) .

NCBI Gene ID

55016

Swiss Prot

Q8TCQ1

Accession Number

Q8TCQ1

Host

Rabbit

Reactivity

Mouse

Immunogen

This MARCH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the Central region of human MARCH1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Immunology

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

32 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

MARCH1

NCBI Official Name

E3 ubiquitin-protein ligase MARCH1

NCBI Organism

Homo sapiens

Background Reference 01

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Background Reference 02

Thibodeau, J., et al. Eur. J. Immunol. 38 (5) :1225-1230 (2008)

Background Reference 03

De Gassart, A., et al. Proc. Natl. Acad. Sci. U.S.A. 105 (9) :3491-3496 (2008)

Background Reference 04

Bartee, E., et al. J. Virol. 78 (3) :1109-1120 (2004)

Other Product Names

E3 ubiquitin-protein ligase MARCH1, 632-, Membrane-associated RING finger protein 1, Membrane-associated RING-CH protein I, MARCH-I, RING finger protein 171, MARCH1, RNF171