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NFYB Antibody

Product Specifications

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.

NCBI Gene ID

4801

Swiss Prot

P25208

Accession Number

P25208

Host

Rabbit

Reactivity

Human

Immunogen

This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human NFYB.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

23 kDa

Fragment

Rabbit Ig

Symbol

NFYB

NCBI Official Name

Nuclear transcription factor Y subunit beta

NCBI Organism

Homo sapiens

Background Reference 01

Stros, M., et al. Nucleic Acids Res. 37 (7) :2070-2086 (2009)

Background Reference 02

Kang, J.H., et al. J. Cell. Biochem. 104 (4) :1192-1203 (2008)

Background Reference 03

Raju, C.S., et al. Mol. Biol. Cell 19 (7) :3008-3019 (2008)

Background Reference 04

Di Agostino, S., et al. Cancer Cell 10 (3) :191-202 (2006)

Other Product Names

Nuclear transcription factor Y subunit beta, CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B, NF-YB, NFYB, HAP3

Tested Applications

WB, IF

Protein ID

399193

Physical Properties

Liquid

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