VPS4B Antibody

Product Specifications

Background

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq].

NCBI Gene ID

9525

Swiss Prot

O75351

Accession Number

O75351

Host

Rabbit

Reactivity

Human

Immunogen

This VPS4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-332 amino acids from the C-terminal region of human VPS4B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

49 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

VPS4B

NCBI Official Name

Vacuolar protein sorting-associated protein 4B

NCBI Organism

Homo sapiens

Background Reference 01

Morita, E., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (29) :12889-12894 (2010)

Background Reference 02

McDonough, C.W., et al. Hum. Genet. (2009) In press :

Background Reference 03

Bruce, E.A., et al. Virology 390 (2) :268-278 (2009)

Background Reference 04

Inoue, M., et al. Traffic 9 (12) :2180-2189 (2008)

Other Product Names

Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K (+) transport growth defect 1, Protein SKD1, VPS4B, SKD1, VPS42