Mouse Bckdk Antibody

Product Specifications

Background

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex (By similarity) .

NCBI Gene ID

12041

Swiss Prot

O55028

Accession Number

O55028

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This Mouse Bckdk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-188 amino acids from the Central region of mouse Bckdk.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

47 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

Bckdk

NCBI Official Name

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial

NCBI Organism

Mus musculus

Background Reference 01

Pagliarini, D.J., et al. Cell 134 (1) :112-123 (2008)

Background Reference 02

Lee, J., et al. Mol. Cell Proteomics 6 (4) :669-676 (2007)

Background Reference 03

Hutson, S.M. Biochem. J. 400 (1), E1-E3 (2006) :

Background Reference 04

Joshi, M.A., et al. Biochem. J. 400 (1) :153-162 (2006)

Other Product Names

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, Branched-chain alpha-ketoacid dehydrogenase kinase, BCKD-kinase, BCKDHKIN, Bckdk