SF3B1 Antibody

Product Specifications

Background

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP) . The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

NCBI Gene ID

23451

Swiss Prot

O75533

Accession Number

O75533

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This SF3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-402 amino acids from the N-terminal region of human SF3B1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Xenopus

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

146 kDa

Fragment

Rabbit Ig

Symbol

SF3B1

NCBI Official Name

Splicing factor 3B subunit 1

NCBI Organism

Homo sapiens

Background Reference 01

Corsini, L., et al. J. Biol. Chem. 284 (1) :630-639 (2009)

Background Reference 02

Tanuma, N., et al. J. Biol. Chem. 283 (51) :35805-35814 (2008)

Background Reference 03

Pessa, H.K., et al. Proc. Natl. Acad. Sci. U.S.A. 105 (25) :8655-8660 (2008)

Background Reference 04

Kuwasako, K., et al. Proteins 71 (4) :1617-1636 (2008)

Other Product Names

Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, SF3b155, Spliceosome-associated protein 155, SAP 155, SF3B1, SAP155

Tested Applications

WB, IHC-P

Physical Properties

Liquid

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