POU3F3 Antibody

Product Specifications

Background

POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences (summarized by Sumiyama et al., 1996 [PubMed 8703082]) .[supplied by OMIM].

NCBI Gene ID

5455

Swiss Prot

P20264

Accession Number

P20264

Host

Rabbit

Reactivity

Human

Immunogen

This POU3F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human POU3F3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Zebrafish, Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

50 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

POU3F3

NCBI Official Name

POU domain, class 3, transcription factor 3

NCBI Organism

Homo sapiens

Background Reference 01

Wissmuller, S., et al. Nucleic Acids Res. 34 (6) :1735-1744 (2006)

Background Reference 02

Kuhlbrodt, K., et al. J. Biol. Chem. 273 (26) :16050-16057 (1998)

Background Reference 03

Sumiyama, K., et al. Mamm. Genome 9 (2) :180-181 (1998)

Background Reference 04

Malik, K.F., et al. Brain Res. Mol. Brain Res. 45 (1) :99-107 (1997)

Other Product Names

POU domain, class 3, transcription factor 3, Brain-specific homeobox/POU domain protein 1, Brain-1, Brn-1, Octamer-binding protein 8, Oct-8, Octamer-binding transcription factor 8, OTF-8, POU3F3, BRN1, OTF8