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PAPOLG Antibody

Product Specifications

Background

This gene encodes a member of the poly (A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly (A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor) /AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14.

NCBI Gene ID

64895

Swiss Prot

Q9BWT3

Accession Number

Q9BWT3

Host

Rabbit

Reactivity

Human

Immunogen

This PAPOLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 685-714 amino acids from the C-terminal region of human PAPOLG.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=56-905

Calculated Molecular Weight

83 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PAPOLG

NCBI Official Name

Poly (A) polymerase gamma

NCBI Organism

Homo sapiens

Background Reference 01

Lee, Y.S., et al. J. Biol. Chem. 285 (36) :28105-28116 (2010)

Background Reference 02

Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101 (33) :12130-12135 (2004)

Background Reference 03

Kyriakopoulou, C.B., et al. J. Biol. Chem. 276 (36) :33504-33511 (2001)

Background Reference 04

Topalian, S.L., et al. Mol. Cell. Biol. 21 (16) :5614-5623 (2001)

Other Product Names

Poly (A) polymerase gamma, PAP-gamma, Neo-poly (A) polymerase, Neo-PAP, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme, PAPOLG, PAP2, PAPG

Tested Applications

WB

Protein ID

116242699

Physical Properties

Liquid

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