RPS12 Antibody

Product Specifications

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

NCBI Gene ID

6206

Swiss Prot

P25398

Accession Number

P25398

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This RPS12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human RPS12.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Pig, Rat, Xenopus

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

15 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

RPS12

NCBI Official Name

40S ribosomal protein S12

NCBI Organism

Homo sapiens

Background Reference 01

Yu, Y., et al. Protein Sci. 14 (6) :1438-1446 (2005)

Background Reference 02

Andersen, J.S., et al. Nature 433 (7021) :77-83 (2005)

Background Reference 03

Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :

Background Reference 04

Sampath, P., et al. Mol. Cell. Biol. 23 (5) :1509-1519 (2003)

Other Product Names

40S ribosomal protein S12, RPS12

Tested Applications

Protein ID

224471878

Physical Properties

Liquid

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