GCAT Antibody

Product Specifications

Background

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14.

NCBI Gene ID

23464

Swiss Prot

O75600

Accession Number

O75600

Host

Rabbit

Reactivity

Human

Immunogen

This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-128 amino acids from the N-terminal region of human GCAT.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

45 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

GCAT

NCBI Official Name

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial

NCBI Organism

Homo sapiens

Background Reference 01

Jacquot, C., et al. Anticancer Res. 22 (4) :2229-2235 (2002)

Background Reference 02

Edgar, A.J., et al. Eur. J. Biochem. 267 (6) :1805-1812 (2000)

Background Reference 03

Tressel, T., et al. J. Biol. Chem. 261 (35) :16428-16437 (1986)

Other Product Names

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL

Tested Applications

Physical Properties

Liquid

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

HY-N12790 1 Each

Arisanschinin D

Ask

View Details

GR131048 100 µg

Genorise® Mouse TGFb1 Polyclonal Antibody

Ask

View Details

TS425554P5 25 Slides

GTF3A CytoSection

Ask

View Details

048121 100 µg

HLA-DR (IMMU-114) discontinued

Ask

View Details

AM8637b 200 µL

Cry2 Antibody

Ask

View Details

TRC-D491773-100MG 100 mg

1,2-(Diphenyl-d10)hydrazine

Ask

View Details